1624
Richard Cornish of the Virginia Colony is tried and hanged for sodomy.
1620 – 1725
Approximately 350 persons are accused of witchcraft in colonial New England, of who 78 percent are women and a number of those thought to have been lesbians. In total, 35 people are executed, 28 of whom are women. More than half of the accusations, trials and executions took place in the town of Salem, Massachusetts, from 1692-1693.
1641– 42
The Massachusetts Bay Colony becomes the first of several New England colonies to incorporate into its laws the language of Leviticus 20:13: `if a man lieth with mankinde, as he lieth with a woman, both of them have committed abomination, they both shall surely be put to death.
1649
The first known conviction for lesbian activity in North America occurs in March when Sarah White Norman is charged with "lewd behavior" with Mary Vincent Hammon in Plymouth, Massachusetts.